The effect of transglutaminase as a binding agent in mixture of meat and non-meat gels on water holding properties at different cooking conditions

RAUJ Marapana1*,SA Senanayaka 1, PRD Perera 1 and Bo Jiang 2

1Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Sri Lanka
2Key Laboratory of Food Science and Safety, Southern Yangtze University, 170 Huihe Road, Wuxi, Jiangsu Province, P. R. China 214036

Abstract

The influence of cooking methods (water bath, retort, and microwave) on the binding and water holding properties of mixed pork/casein gels with (2.5 or 5 gkg-1) or without (control) microbial transglutaminase (MTGase) was investigated using puncture test measurements. The MTGase treatment improved (P< 0.0001) water-holding capacity, reduced P<0.0001) cooking loss (CL), purge and expressible water (EW), and increased (P<0.0001) the gel hardness and breaking strength as measured by compression and puncture tests. Microwave-cooked mixed gels had the lowest hardness but least CL and EW than retort and water bath-cooked samples. The results suggested that MTGase influenced the gel properties of pork muscle/non muscle gels, but the effectiveness of the enzyme treatment depended on the cooking methods.

Key words: Meat, Casein, MTGase, Cooking Method, Water holding, Gel strength

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* - Corresponding Author

Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka

Copyright © 2007 by the Faculty of Agriculture, University of Ruhuna

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